Sister chromatid exchange analysis in some Holstein bulls
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Abstract:
Chromosomal appearance of 12 Holstein bulls selected for artificial inseminations were examined bysister chromatid exchanges (SCEs). For differential staining of sister chromatids bromodeoxyuridine (BrdU)was inoculated in lymphocyte cultures. The mean, maximum and minimum number of SCE per cell weredetermined 6.8 ± 1.14, 8.3 ± 1.1 and 5.7 ± 1.5, respectively. SCE frequencies of all animals were in thenormal level. Also, evaluation of different breeding condition and age range did not show any significantstatistical (P>0.05) effect on SCEs rates
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sister chromatid exchange analysis in some holstein bulls
chromosomal appearance of 12 holstein bulls selected for artificial inseminations were examined bysister chromatid exchanges (sces). for differential staining of sister chromatids bromodeoxyuridine (brdu)was inoculated in lymphocyte cultures. the mean, maximum and minimum number of sce per cell weredetermined 6.8 ± 1.14, 8.3 ± 1.1 and 5.7 ± 1.5, respectively. sce frequencies of all animals were...
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A fully optimized staining method for detecting sister chromatid exchanges in cultured cells is presented. The method gives reproducibly robust quantitative results. Sister chromatid exchange is a classic toxicology assay for genotoxicity and for detecting alterations to the biochemistry underlying cellular homologous recombination. Growth of cells in the presence of 5'-bromo-deoxyuridine for t...
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Journal title
volume 13 issue 2
pages 161- 163
publication date 2012-06-01
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